Pierre David

Transgenesis Platform

Of all the mammals studied, mouse became the model of choice for several reasons. Its genome is known since 2002 and we share approximately 95 % of our genomes. Moreover, is has a small size and relatively rapid generation time.

Mouse is an important tool for understanding gene function, researching human diseases, or testing responses to a therapeutic intervention.

New transgenesis techniques have emerged as powerful new tools for genome modifications, especially Crispr/Cas9 system and this is what the Federative Research Structure – Necker aims to develop.

 

Our main mission is the generation of genetically modified mice with Crispr/Cas9 system.

The plateform will provide :

  • Embryo production (available strains : C57Bl/6J, FVB/N, B6D2F1)
  • Zygote injection at pronuclear stage with Crispr/Cas9 components
  • Transfer of the injected embryos in pseudopregnants females
  • Tail biopsy of the newborn pups

 

The Transgenesis Platform is located in the Animal Experimentation and Transgenesis Laboratory (LAET) in the Imagine Institute.

 
Equipment :
- A CO2 incubator
- A -80°C freezer
- A Leica DMIRB inverted microscope
- An Eppendorf NK2 micromanipulators
- An Eppendorf Femtojet micro-injectors
- 2 stereomicroscopes