ENGINEER POSITION (F/M)

The "Laboratory of Mechanisms and Therapy of Genetic Brain Diseases”, directed by Dr Michela Deleidi at the Imagine Institute in Paris (15) is looking for an assistant engineer for 24 months (minimum).

Imagine Institute

Imagine Institute, on the Necker-Enfants Malades Hospital campus in Paris, is an interdisciplinary biomedical research centre, providing excellent core facilities for genomics, cell imaging, and bioinformatics together with creating animal models of human disease (mice, Zebrafish, and Drosophila). Additionally, Imagine strongly emphasizes translational aspects and innovative therapeutic options, including gene therapies. Imagine, supported by a private Foundation, is affiliated with INSERM (French national institute for medical research, U1163), Université Paris Cité and AP-HP(Paris Public Hospitals Group). As a research and healthcare Institute, Imagine focuses on a wide variety of genetic diseases, studying all aspects from basic research to translational medicine with an ultimate aim of developing new approaches to therapy including gene therapy and gene editing. The Institute includes over 600 staff members in 28 laboratories organized in integrated care and research programs addressing genetics, immunology and infectious diseases, haematology, nephrology, developmental defects, neurosciences and bioinformatics.

The "Laboratory of Mechanisms and Therapy of Genetic Brain Diseases”, directed by Dr Michela Deleidi at Imagine Institute in Paris 15 is looking for an assistant engineer.

The laboratory's projects aim at dissecting the molecular mechanisms of genetic brain diseases, with a focus on the link between rare mendelian diseases and age-associated disorders. The laboratory focuses on unique and shared molecular pathways underlying inflammatory, infectious, and neurodegenerative diseases. Another line of research in our laboratory aims at investigating the risk/resilience factors of neuronopathic lysosomal storage diseases. To this end, we develop models with human pluripotent stem cells and long-term cultures of organoids combined with single cell-omics to identify the role of disease related genetic variants and validate targets with diagnostic and therapeutic potential.

MISSION AND FUNCTIONS

The candidate will participate in various research projects relating to the understanding of the physiopathology of genetic brain diseases, using cellular models of iPSCs cells, which are subsequently differentiated in organoids (brain, intestinal). Activities will include:

  • Performing maintenance of iPSCs
  • Implementing organoid differentiation protocols and perform initial characterization
  • Developing and conduct experiments in molecular biology, cell biology and biochemistry
  • Implementing imaging techniques (for example, confocal microscopy)
  • Lab management responsibilities including purchasing laboratory reagents, monitoring consumable spending, negotiating with vendors for the purchase of lab equipment and bulk quantity items

Weekend on-call duty may be required as needed.

REQUIREMENTS

The successful candidate must have a strong background in neuroscience with knowledge of molecular and cell biology and previous experience in pluripotent stem cell culture and differentiation. Preference will be given to candidates with additional strong background in molecular biology and CRISPR/Cas9 gene editing. The ability to work independently, establish new techniques, interpret and discuss experiments is essential, together with excellent organizational skills. We are looking for a team- and goal-oriented personality. Fluency in scientific English. Masters level, Engineering School.

POSITION

The position is for 2 years with a possible extension. The employment is planned to start in March 2023 or upon agreement with the chosen candidate. Fixed term contract for 24 months, possibility of extension. Salary according to experience

Location: Institut des Maladies Génétiques Imagine – 24 Boulevard du Montparnasse – 75015 Paris

Applications in English or French including a cover letter, a detailed CV with publication list and contact information of two references should be sent to michela.deleidi@institutimagine.org