Nathalie Lefort

Imagine Institute iPSC core facility

In July 2015, the Imagine Institute iPSC Core Facility was created. The iPSC platform allows the development of new cellular models for the study of rare diseases. The goals of the platform are three-fold:

• The generation and maintenance of iPS cells
• The characterization of iPS Cell lines
• Users training

The missions of the Platform are :

• Cellular reprogramming of human somatic cells from patients and healthy donors into iPS cells
• Expanding, maintaining, and banking of iPS cells
• Molecular and functional characterization of iPS cells
• Scientific and technical advice to users
• Assist users in the design of their project

Equipment

The iPSC platform is equipped with:

• Three incubators including 1 with tris-gas option for both hypoxic and hyperoxic studies
• Three class II biological safety cabinets
• Inverted microscope with phase contrast optics
• Two Lynx Dynascope under class II biological safety cabinet
• Celena™ S Digital Cell Imaging System
• Orbital shaker
• Centrifuge
• Ultra low temperature freezer (-150°C)