Pierre David

Of all the mammals studied, mouse became the model of choice for several reasons. Its genome is known since 2002 and we share approximately 95 % of our genomes. Moreover, is has a small size and relatively rapid generation time.

Mouse is an important tool for understanding gene function, researching human diseases, or testing responses to a therapeutic intervention.

New transgenesis techniques have emerged as powerful new tools for genome modifications, especially Crispr/Cas9 system and this is what the Federative Research Structure – Necker aims to develop.

Our main mission is the generation of genetically modified mice with Crispr/Cas9 system.

The plateform will provide :

• Embryo production (available strains : C57Bl/6J, FVB/N, B6D2F1)
• Zygote injection at pronuclear stage with Crispr/Cas9 components
• Transfer of the injected embryos in pseudopregnants females
• Tail biopsy of the newborn pups

The Transgenesis Platform is located in the Animal Experimentation and Transgenesis Laboratory (LAET) in the Imagine Institute.