Pierre David

Plateforme de transgenèse

Publié le 20.11.2019

Présentation

Of all the mammals studied, mouse became the model of choice for several reasons. Its genome is known since 2002 and we share approximately 95 % of our genomes. Moreover, is has a small size and relatively rapid generation time.

Mouse is an important tool for understanding gene function, researching human diseases, or testing responses to a therapeutic intervention.

New transgenesis techniques have emerged as powerful new tools for genome modifications, especially Crispr/Cas9 system and this is what the Federative Research Structure – Necker aims to develop.

 

Our main mission is the generation of genetically modified mice with Crispr/Cas9 system.

The plateform will provide :

  • Embryo production (available strains : C57Bl/6J, FVB/N, B6D2F1)
  • Zygote injection at pronuclear stage with Crispr/Cas9 components
  • Transfer of the injected embryos in pseudopregnants females
  • Tail biopsy of the newborn pups

The Transgenesis Platform is located in the Animal Experimentation and Transgenesis Laboratory (LAET) in the Imagine Institute.

 

Equipment

 
  • A CO2 incubator
  • A -80°C freezer
  • A Leica DMIRB inverted microscope
  • An Eppendorf NK2 micromanipulators
  • An Eppendorf Femtojet micro-injectors
  • 2 stereomicroscopes

Ressources & publications

Cellules du colon

La recherche : une aventure scientifique

Notre objectif : mieux comprendre les maladies génétiques pour mieux les soigner.