Christine Bole-Feysot

Plateforme de génomique

Publié le 19.11.2019

Présentation

Plateforme Génomique

The Imagine’s genomics core facility was created in 2008 to provide high-throughput genotyping, sequencing and gene expression services to the Necker research community on a fee-for-service basis. Experimental design and the, analysis and interpretation of results are performed on an interactive basis with investigators and the bioinformatics facility (Imagine Foundation/Paris Descartes University). Available Equipments and services The genomic platform is equipped with :

  • One high troughtput next generation sequencer NovaSeq, Illumina
  • One high troughtput next generation sequencer iSeq100, Illumina
  • Other equipments :
  1. DNA shearing: Covaris E220
  2. Capillary electrophoresis: Fragment Analyseur et Tape Station 2200 Agilent Technologies
  3. Nucleic acids measurement: Xpose spectrophotometer (Unchained), QuBit fluorimeter (Invitrogen), real time PCR StepOnePlus (Life Technologies)

Services provided The core facility provides :

  • An help to the experimental design
  • The quality check of DNAs and RNAs samples
  • The library construction, clonal amplification and sequencing with next generation sequencers
  • The primary data analysis and transfer to the bioinformatics facility for further data analysis

Proposed applications :

  • Targeted re-sequencing: exome sequencing mainly and other types of targeted re-sequencing
  • RNA-Seq librairies and sequencing
  • Whole genome Sequencing
  • Copy Number Variation analysis and association studiesOther applications of next generation sequencing: Sequencing of ChIP-Seq (sequencing of immunoprecipitated chromatin), ATAQ-Seq, Repli-Seq, amplicons, Single-cell and other types of applications
Cellules du colon

La recherche : une aventure scientifique

Notre objectif : mieux comprendre les maladies génétiques pour mieux les soigner.

Chiifres clé

    10000 Exomes

    1600 RNA-Seq