Christine Bole-Feysot

The Imagine’s genomics core facility was created in 2008 to provide high-throughput genotyping, sequencing and gene expression services to the Necker research community on a fee-for-service basis. Experimental design and the, analysis and interpretation of results are performed on an interactive basis with investigators and the bioinformatics facility (Imagine Foundation/Paris Descartes University). Available Equipments and services The genomic platform is equipped with :

• One high troughtput next generation sequencer NovaSeq, Illumina
• One high troughtput next generation sequencer iSeq100, Illumina
• Other equipments :

1. DNA shearing: Covaris E220
2. Capillary electrophoresis: Fragment Analyseur et Tape Station 2200 Agilent Technologies
3. Nucleic acids measurement: Xpose spectrophotometer (Unchained), QuBit fluorimeter (Invitrogen), real time PCR StepOnePlus (Life Technologies)

Services provided The core facility provides :

• An help to the experimental design
• The quality check of DNAs and RNAs samples
• The library construction, clonal amplification and sequencing with next generation sequencers
• The primary data analysis and transfer to the bioinformatics facility for further data analysis

Proposed applications :

• Targeted re-sequencing: exome sequencing mainly and other types of targeted re-sequencing
• RNA-Seq librairies and sequencing
• Whole genome Sequencing
• Copy Number Variation analysis and association studiesOther applications of next generation sequencing: Sequencing of ChIP-Seq (sequencing of immunoprecipitated chromatin), ATAQ-Seq, Repli-Seq, amplicons, Single-cell and other types of applications