Christine Bole-Feysot

The Imagine’s genomics core facility was created in 2008 to provide high-throughput genotyping, sequencing and gene expression services to the Necker research community on a fee-for-service basis. Experimental design and the, analysis and interpretation of results are performed on an interactive basis with investigators and the bioinformatics facility (Imagine Foundation/Paris Descartes University).

Available Equipments and services

The genomic platform is equipped with :

• Two high troughtput next generation sequencers HiSeq2500, Illumina
• One next generation sequencer Ion Torrent PGM (Personal Genome Machine), Life Technologies
• A GeneChip microarray plateform, Affymetrix
• Other equipments :
  • DNA shearing: Covaris S2, Covaris E220, Hydroshear, GeneMachines
  • Capillary electrophoresis: Bioanalyseur 2100 et Tape Station 2200 Agilent Technologies
  • Nucleic acids measurement: Nanodrop ND-1000 spectrophotometer (Thermo Scientific), QuBit fluorimeter (Invitrogen), real time PCR StepOnePlus (Life Technologies)

Services provided

The core facility provides :

• An help to the experimental design
• The quality check of DNAs and RNAs samples
• The nucleic acids amplification, labeling and hybridization on Affymetrix microarrays
• The library construction, clonal amplification and sequencing with next generation sequencers
• The primary data analysis and transfer to the bioinformatics facility for further data analysis

Proposed applications :

• Targeted re-sequencing: exome sequencing mainly and other types of targeted re-sequencing
• Gene expression profiling in man and mouse
• Linkage analysis, Copy Number Variation analysis and association studies
• Other applications of next generation sequencing: ChIP-Seq (sequencing of immunoprecipitated chromatin), sequencing of amplicons and other types of applications